Methodology for the formation of an extractive agent of polymerized carbon nanotubes encapsulating phenol nanoparticles used for DNA extraction and purification, and product thereof

摘要

A method for synthesis of nanocomposites using polymerized carbon nanotubes and phenolic compounds, such as 2,4-dichlorophenol, 4-nitrophenol, 2-nitrophenol, 4-chlorophenol, 2,4,6-trinitrophenol and phenol, in aqueous media, as well as the application of one of such nanocomposites, namely MWCNT-g-PCA-Phenol, as a DNA extraction agent. The aforementioned nanocomposites enable the isolation of genomic DNA of plant samples. Pursuant to the present invention, the toxicity of free phenols used in DNA extraction is decreased since very low concentrations of phenol are required in the method, as compared to conventional techniques which employ saturated phenol solutions. This remarkable advantage is due to the encapsulation of the phenol nanoparticles into the polymerized carbon nanotubes.