Method of purifying a fusion protein - Fc fusion proteins containing purified FC, TNFR: FC, purified, and TNFR: FC elevadamente purified

摘要

The invention relates generally to methods for purifying a Fc-fusion protein produced in a eukaryotic expression system. More specifically, the invention provides a robust and scalable downstream purification process suitable for use in manufacturing TNFR:Fc for human administration which comprises an optimized Protein A affinity chromatography step and two ion exchange chromatography steps both of which are operated in the bind-and-elute mode.