high-yield transient expression in mammalian cells using unique high density growth-matched transfection medium and expression enhancers

摘要

"High-yield transient expression in mammalian cells using exclusive high density growth-matched transfection medium and expression enhancers" The present invention is generally directed to cell culture media (particularly serum free, non-animal derived media) , and / or chemically defined media) which are useful for introducing macromolecules and compounds (e.g. nucleic acid molecules) into cells (e.g. eukaryotic cells). According to the invention, this introduction may occur in the presence of said medium. cells containing such introduced materials can then be cultured in the medium and the effect of the introduced materials on cells can be measured or determined. In particular, the invention allows the introduction of nucleic acid molecules (e.g., vectors) into cells (particularly eukaryotic cells) and the expression of proteins encoded by nucleic acid molecules in cells. The present invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells (e.g., cell culture versus cell transfection). The invention also relates to compositions and kits useful for cell culture and transformation / transfection.