Somatic cell genetically modified, method for producing a non-human animal, and an animal other than human cloned oocyte

摘要

1 / 1 Summary u00e2u20acu0153cu00e3u2030lula somu00e3ufffdtica genetically modified, mu00e3u2030todo to produce an animal nu00e3u0192o human, and,Ovu00e3 "quote of a cloned animal nu00e3u0192o humanou00e2u20acufffd cu00e3lulas somu00e3ticas genetically modified animal human nu00e3u00a3o su00e3u00a3o provided that su00e3u00a3o designed to contain a tape of expressu00e3u00a3o recombinase autoexcisu00e3vel comprising a gene recombinase su00e3u00adtio - especu00e3u00adfico oper Acionalmente linked to a promoter especu00e3u00adfico of cu00e3lula es.Composiu00e3u00a7u00e3u00b5es and mu00e3todos to produce an animal human nu00e3u00a3o genetically modified cloned that is free of a selective marker gene and a gene recombinase su00e3u00a3o provided, in which a targeting construct comprising a gene recombinase operationally connected to autoexcisu00e3vel A promoter especu00e3u00adfico of cu00e3lula es is introduced in cu00e3lulas somu00e3ticas differentiated.The genome of the genetically modified cu00e3lulas somu00e3ticas transferred in a ovu00e3u00b3cito host without nu00e3u00bacleo. The zygote, artificially created the entu00e3u00a3o grown in vitro controllers the estu00e3gio embrionu00e3rio of blastocyst and subsequently implanted in a u00e3u00batero a mu00e3u00a3e rent to form an animal nu00e3u00a3o genetically modified cloned human free and recombinase selective marker gene.