AN ANTI-CANCER ACTIVE SUBSTANCE FROM ANTRODIA CAMPHORATA, METHOD FOR PREPARING THE SAME AND USE THEREOF

摘要

AN ANTI-CANCER ACTIVE SUBSTANCE FROM ANTRODIA CAMPHORATA, PRODUCED FROM MYCELIUM OF ANTRODIA CAMPHORATA WITH FOLLOWING STEPS: (1) CULTURING MYCELIUM OF ANTRODIA CAMPHORATA BY LIQUID FERMENTATION; (2) EXTRACTING THE THUS-OBTAINED MYCELIUM WITH ETHANOL AND CONCENTRATING THE EXTRACTED SOLUTION TO OBTAIN AN ETHANOL EXTRACT; (3) DISSOLVING SAID ETHANOL EXTRACT IN WATER, AND EXTRACTING WITH EQUAL VOLUME OF ETHYL ACETATE TO OBTAIN ETHYL ACETATE EXTRACT; (4) PURIFYING SAID ETHYL ACETATE EXTRACT BY SILICA GEL COLUMN CHROMATOGRAPHY USING HEXANE/ETHYL ACETATE GRADIENT AS THE MOBILE PHASE TO OBTAIN AN ELUATE FRACTION G; AND (5) PURIFYING SAID ELUATE FRACTION G BY SILICA GEL COLUMN CHROMATOGRAPHY USING HEXANE/ETHYL ACETATE GRADIENT AS THE MOBILE PHASE TO OBTAIN A SPECIFIC ELUATE FRACTION WHICH COULD BE PURIFIED FURTHER TO OBTAIN SAID ANTI-CANCER ACTIVE SUBSTANCE FROM ANTRODIA CAMPHORATA.