HUMANIZED LACTATE DEHYDROGENASE (LDH) SACCHAROMYCES CEREVISIAE AND CONSTRUCTION METHOD THEREOF

摘要

Provided are a humanized lactate dehydrogenase (LDH) Saccharomyces cerevisiae and the construction method thereof. In particular, the method of gene homologous recombination is used to knockout the genes PDC1, PDC5 and PDC6 which encode the PDC of Saccharomyces cerevisiae, LDH from humans is expressed in Saccharomyces cerevisiae, and antimycin A is used to block electronic chain transfers, thus making humanized lactic acid fermentation replace ethanol fermentation for maintaining glycolysis. Humanized lactate dehydrogenase (LDH) Saccharomyces cerevisiae obtained from the present method can express the lactate dehydrogenase (LDH) of humans. LDHA or LDHC is expressed in strains of Saccharomyces cerevisiae suffering from three deficiencies in pdc1Δ pdc6Δ pdc5Δ. After antimycin A is added, the strains can grow, and the production of lactic acid can be detected.