Provided are parthenogenetic haploid embryonic stem cells, a preparation method therefor, and application of the parthenogenetic haploid embryonic stem cells in constructing gene modification semi-cloned animals and a gene modification semi-cloned animal library. H19-DMR and IG-DMR of the parthenogenetic haploid embryonic stem cells are knocked out. The parthenogenetic haploid embryonic stem cells are capable of stably obtaining survival SC mice by being injected in ova, and can be effectively used for polygenic inheritance operation, thereby further obtaining animals carrying polygenic inheritance modification.
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