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METHODS AND KITS FOR TRACKING NUCLEIC ACID TARGET ORIGIN FOR NUCLEIC ACID SEQUENCING
METHODS AND KITS FOR TRACKING NUCLEIC ACID TARGET ORIGIN FOR NUCLEIC ACID SEQUENCING
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机译:核酸测序的核酸目标溯源方法和试剂盒。
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摘要
The present disclosure provides methods and kits for tracking nucleic acid target origin by barcode tagging of the targets when they break into smaller fragments. Nucleic acid targets are captured in vitro by clonally localized nucleic acid barcode templates on a solid support. Millions of nucleic acid targets can be processed simultaneously in a massively parallel fashion without additional partition. These captured targets are broken into small fragments, and a target specific barcode sequence is tagged on each fragment as an identification of their original target. These nucleic acid target tracking methods can be used for a variety of applications in both whole genome sequencing and targeted sequencing.
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