METHOD FOR DECELLULARIZING BRAIN TISSUE AND METHOD FOR CULTURING BRAIN TISSUE

摘要

The present invention relates to a decellularizing method and a culturing method of brain tissue, and more specifically, provides a method for decellularizing brain tissue, which comprises the following steps: a step for providing the brain tissue which becomes a target to be decellularized; a first cell removing step of removing cells of the tissue at the temperature equal to or lower than the room temperature by using a solution containing a first surfactant; a DNA degrading step of decomposing DNA of tissue treated in the first cell removing step by using a solution containing a DNA degrading enzyme; and a second cell removing step of removing cells of the tissue treated in the DNA degrading step at the temperature equal to or lower than room temperature by using a solution containing a second surfactant, and a method for culturing brain tissue using the same.;COPYRIGHT KIPO 2017