MUTANT METHYLGLYOXAL SYNTHASE MGS FOR THE PRODUCTION OF A BIOCHEMICAL BY FERMENTATION

摘要

The present invention encompasses culturing modified microorganisms for improved production of biochemicals selected from lactic acid, acetol and 1,2-propanediol in a suitable culture medium, and recovering the desired biochemical material that can be further purified , Wherein the microorganism expresses a methylglyoxal synthase (MGS) enzyme whose activity is not inhibited by orthophosphate (MGS), a method for producing a biochemical selected from lactic acid, acetol and 1,2-propanediol . The present invention encompasses one or more amino acid residues in the protein sequence of a parent enzyme substituted with a different amino acid residue at the same position wherein the mutant enzyme retains the methylglyoxal synthase activity of the parent enzyme in excess of 50% , - mutant methylglyoxal synthase wherein the methylglyoxal synthase activity of mutant MGS is not inhibited by orthophosphate as compared to the parent enzyme.