Method for assessing periodontal colonization by pathogenic bacteria using real-time polymerase chain reaction

摘要

A method for assessing periodontal colonization by pathogenic bacteria, characterized in that the total DNA is isolated from periodontal flushing, examined by the method of real-time polymerase chain reaction with primers and probes on Prevotella intermedia, Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Tannerella for each reaction, Tannerella for each and 3 the absolute value of the threshold cycle Ct is automatically calculated, after which the signal is normalized, at which the relative Ct value is determined for each sample, for this, from the value of the absolute threshold Ikla Ct for the sequence-specific primer set and probe subtracted value threshold cycle Ct total bacterial mass; pathological colonization of the periodontium is recorded provided that the relative Ct is 15.0 cycles or less.