METHOD UTILIZING ENZYME SUBSTRATES PRODUCING INSOLUBLE FLUORESCENT PRODUCTS AND CHROMOGENS, AND USE IN COMBINATION WITH ENZYME SUBSTRATES PRODUCING SOLUBLE PRODUCTS

摘要

A method of detection of cells, microorganisms, or molecules by the use of various combinations of fluorogens and a chromogens which yield fluorophores and chromophores when cleaved by specific enzymes and which can be viewed by UV and visible light from both sides of an absorbent pad. Included is the method of application of a family of compounds producing both insoluble fluorophores and chromophores identified as dual enzyme substrates.