Use of vitamins and vitamin metabolism-related genes and proteins for recombinant protein production in mammalian cells

摘要

Disclosed are eukaryotic expression systems and methods for selection of mammalian cell lines that produce a protein of interest, such as a therapeutic protein. These systems and methods allow simple and rapid selection of cells that mediate high levels of recombinant protein production. These systems and methods reduce the cost and cost of therapeutic proteins by reducing the effort and time required to bring new therapeutic proteins to the patient and increasing the ability of cells to produce in the bioreactor Let [Selection] Figure 1