Method for evaluating the effect of a test substance or an article on the skin

摘要

A contacting step of maintaining a state in which the test substance or article is in contact with human skin for a predetermined period of time, A step of obtaining a cross-sectional image of the stratum corneum at an arbitrary depth of the skin following the contacting step, a step of acquiring a post- Acquiring the area of the hillock area and the area of the opening in the hillock area from the cross sectional image of the stratum corneum acquired in the above-described contacting state acquiring step, information acquiring process of the hill region after contacting, An opening area ratio calculating step of calculating a ratio of the area of the opening portion to the area of the ridge area,A method for evaluating the effect of a test substance or article on barrier function of human skin.