Method for producing collagen degrading enzyme and method for producing collagen tripeptide using the same

摘要

A method for producing a collagenolytic enzyme of the present invention comprises a first step of centrifuging a Bacillus subtilis strain, a second step of concentrating a centrifuged supernatant, a step of purifying by using an ion exchange chromatography method 3, wherein the method for producing a collagen tripeptide comprises a first step of mixing the pretreated fish scales with water at a weight ratio of 2: 8, and heat treating the mixture at 90 C. for 5 hours A second step, a third step of introducing a collagenolytic enzyme produced by the above-mentioned method and decomposing at a temperature of 35 C. for 12 hours, a third step of decomposing the component from the third step through a centrifugal separation, A fifth step of purifying said components by ion exchange chromatography, a sixth step of concentrating said purified components, a seventh step of purifying said concentrated components with activated carbon , Characterized in that it comprises an eighth step of sterilization of the components in the filter.