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SMALL-INTERFERING RNA EXPRESSION SYSTEMS FOR PRODUCTION OF SMALL-INTERFERING RNAS AND THEIR USE
SMALL-INTERFERING RNA EXPRESSION SYSTEMS FOR PRODUCTION OF SMALL-INTERFERING RNAS AND THEIR USE
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机译:用于生产小干扰RNA的小干扰RNA表达系统及其用途
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摘要
A method of preparing a library of small interfering RNA (siRNA) expression systems for producing siRNA for silencing of target genes by inducing degradation of target gene RNA expression products includes: (i) isolating RNA of one or more target genes from a cell population; (ii) generating RNA fragments from the isolated RNA; (iii) converting the RNA fragments into dsDNA fragments; and (iv) cloning the dsDNA fragments into vectors for forming cloned vectors, each vector including one or more promoters and at least one restriction enzyme site capable of accepting the insertion of at least one dsDNA fragment such that siRNA can be produced. Methods for producing siRNA from the siRNA expression system and methods of identifying a functional target gene for treatment by using the siRNA produced from the siRNA expression system and for identifying RNAi therapeutics are also provided.
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