An apparatus and methods of using the same for conducting photonic and optical treatments on biological samples with additional functions including temperature monitoring and real-time microscopic imaging are provided. The photonic and optical treatments can be conducted using light with wavelengths in the near-infrared region (NIR) on biological samples, including in-vitro brain cell cultures, in-vivo central nervous system (CNS) and peripheral nervous system (PNS) tissue samples, and other body tissues. The apparatus and methods can be combined with magnetic nanoparticles treatment to accomplish non-invasive, on-demand drug targeting, brain cell specific gene delivery, and magnetized photo-biomodulation for treating various CNS disorders.
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