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Substrate replenishment and byproduct removal improve yeast cell-free protein synthesis
Substrate replenishment and byproduct removal improve yeast cell-free protein synthesis
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机译:底物补给和副产物去除改善了酵母无细胞蛋白质的合成
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摘要
Methods and kits are provided for calibrating a cell-free protein synthesis reaction for optimal activity. The method includes the steps of providing an extract competent for cell-free protein synthesis (CFPS); performing cell-free protein synthesis with the extract; measuring a first reaction end-point where in vitro protein synthesis plateaus; measuring a second reaction end-point where Energy Charge of the extract declines to a level in a range from about 0.40 to about 0.80 of Energy Charge of a control extract; and adjusting Energy Charge of the extract to a level in a range from about 0.80 to about 1.0 of the control extract.
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