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Method for producing isoprene using recombinant halophilic methanotroph

机译:用重组嗜盐甲烷营养菌生产异戊二烯的方法

摘要

The present invention relates to a recombinant methanotroph having an ability to produce isoprene and a method for producing isoprene using the same, and more particularly to a recombinant methanotroph having an ability to produce isoprene wherein a gene encoding an isoprene synthase having a homology of at least 70% to the amino acid sequence of Ipomoea batatas isoprene synthase is introduced into the recombinant methanotroph, and a method for producing isoprene using the recombinant methanotroph. The use of a recombinant methanotroph according to the present invention enables isoprene to be produced in high yield by using methane gas or methanol which is obtained from waste such as natural gas, biomass, municipal waste or the like as a carbon source.
机译:具有异戊二烯生产能力的重组甲烷营养体和使用异戊二烯生产异戊二烯的方法技术领域本发明涉及一种具有异戊二烯生产能力的异丁烯营养体,更具体地,涉及一种具有异戊二烯生产能力的重组异甲基营养体,其中编码异戊二烯合酶的基因至少具有同源性。将番薯叶番薯属氨基酸的70%的异戊二烯合酶引入重组甲烷营养菌中,并提出了利用该重组甲烷营养菌生产异戊二烯的方法。通过使用本发明的重组甲烷营养体,通过使用从天然气,生物质,城市废弃物等废弃物中得到的甲烷气体或甲醇作为碳源,可以高收率地生产异戊二烯。

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