A method for identifying a strategy to achieve fertilization in mammals and the right Moment for insemination

摘要

Claim 1: a method to identify a strategy to achieve fertilization in Mammals that includes the following steps: a first sample capacity of sperm CellsAs a Fluorescent marker in vitro; obtaining one or more tu2080 Fluorescent Images that Exhibit one or more patterns of localization of gmu2081 associated with in vitro capacitated Sperm CellsTu2080 marked Fluorescent, fluorescent Images obtained in these moments tu2080 Post Training in vitro selected among: 0.1 hour to 5 hours; 0.1 hour to 8 hours; 0.1 or 0 to 12 hours;18 hours (1 hour tu2080); Measuring a number of Patterns of localization in the acrosome gmu2081 apical (AA)A number of Patterns in the location of gmu2081 acrosomal plasma membrane (APM) and a total quantity of Patterns of localization of gmu2081 trained for Sperm cells in vitro coN Fluorescent Marker tu2080 exhibited fluorescence in the images to determine a percentage of tu2080 localization patterns gmu2081 [aa] More patterns of localization gmu2081 APM is a tu2080; determineOJ Fertility Patterns associated with a percentage of [Location gmu2081 AA more patterns of localization of gmu2081 APM tu2080] where a reference percentage [patterns of localization of gmu2081 AAMost patterns of localization of gmu2081 APM] corresponding to: greater than 35% indicates a State of high fertility; a Standard Deviation below 35% indicates a State of average fertility; and two or more diversionS Standard below 35% indicate a State of Low Fertility rate; compare the localization patterns of gmu2081 [aa] gmu2081 APM Tracing patterns of tu2080 measured as the percentage of referGum [patterns of localization of gmu2081 AA more patterns of localization of gmu2081 APM]; and identify a Reproductive Strategy based on fertility status to achieve the FertilizAtion.