首页> 外国专利> marked gene activation DNA construct, marked population of maize plants, method for generating it and method for generating a marked maize plant

marked gene activation DNA construct, marked population of maize plants, method for generating it and method for generating a marked maize plant

机译:标记的基因激活DNA构建体,玉米植物的标记种群,其产生方法和标记的玉米植物的产生方法

摘要

marked gene activation platform for maize, and resulting labeled populations and plants. The present invention relates to a labeled gene activation construct for maize, the resulting labeled populations and plants. In one example, a tagged gene activation DNA construct includes a transposase coding sequence, a detectable reporter (such as the b-turkey and c1 anthocyanin regulatory genes) and a non-autonomous transposable t-dna cassette. for example, the transposable t-dna cassette is inserted into the detectable reporter coding region so that the b-turkey and c1 genes express anthocyanins in a cell containing the corn labeled gene activation dna construct only by excision of the cassette transposable. Methods for generating the marked population of maize plants include transforming a maize plant cell or tissue with the constructs presented.
机译:标记的玉米基因激活平台,以及标记的种群和植物。本发明涉及用于玉米的标记的基因激活构建体,所得的标记的种群和植物。在一个实例中,标记的基因激活DNA构建体包括转座酶编码序列,可检测的报道分子(例如b-火鸡和c1花色苷调节基因)和非自主的可转座t-dna盒。例如,将可转座的t-dna盒插入到可检测的报道基因编码区,以便仅通过切除可转座的盒,b-火鸡和c1基因在含有玉米标记的基因激活dna构建体的细胞中表达花色苷。产生标记的玉米植物种群的方法包括用所示的构建体转化玉米植物细胞或组织。

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