Method for obtaining conjugate of docetaxel, trastuzumab and dendrimer PAMAM of 4th generation that has 64 amino groups on its surface and the application of docetaxel, trastuzumab and dendrimer PAMAM of 4th generation that has 64 amino groups on its surface for production of a drug intended for treatment of breast cancer

摘要

the object of the application is the way to receive docetaxel conjugate, trastuzumab and dendrymeru pamam generation 4 having surface 64 amino group and application of docetaxeltrastuzumab and dendrymeru pamam generation 4 having surface amino groups to produce 64 your medicine for therapy of breast cancer.way to receive trastuzumab and docetaxel conjugate, dendrymeru pamam generation 4 having amino group on the surface 64, is thatin the first stage of succinic acid molecules undergo modification of hydroxyl groups of docetaxel.next, in the presence of edc (ethyl n - (3-dimethylamino) propyl - n '- etylokarbodiimidu) is formed between the surface dendrymeru binding amide linkages, with the previously modified substrate.the reaction results in dmso at room temperature.in the later stage of reaction, in order to connect to dendrymeru trastuzumab pamam uses adapter molecule n - sukcynoimidylo - 4 - (nmaleimidometylo) cykloheksylokarboksylan - (smcc) holding onits end functional group forming reaction with both tiolami and amines.in addition to the surface dendrymeru is tiolowu0105 - sh group, using trauta reagent (ethyl 2 - iminotiolanu).the modified dendrymeru00f3w pamam accompanied by monoclonal antibody previously sprzu0119gniu0119te hyphenated smcc.