A METHOD FOR ENANTIOSEPARATION OF TRIAZOLE FUNGICIDES

摘要

A METHOD FOR ENANTIOSEPARATION OF TRIAZOLE FUNGICIDES VIA A CYCLODEXTRIN MODIFIED-MICELLAR ELECTROKINETIC CHROMATOGRAPHY (CD-MEKC) METHOD COMPRISES THE STEPS OF INJECTING A MIXTURE OF THE ENANTIOMERS OF THE TRIAZOLE FUNGICIDES INTO A FUSED-SILICA CAPILLARY OF AT LEAST 50 CM EFFECTIVE LENGTH WITH AN INTERNAL DIAMETER OF 50 - 75 µM UNDER A PRESSURE INJECTION OF 25-50 MBAR FOR 0.5 TO 2 SECONDS, AND ELUTING EACH ENANTIOMER OF THE TRIAZOLE FUNGICIDES IN THE MIXTURE AT SEPARATED OCCASIONS CHARACTERIZED IN THAT THE BACKGROUND SOLUTION INCLUDES A PHOSPHATE BUFFER AT A PH 2.5 TO 4.0 OR 7.0 TO 9.0, AN ANIONIC SURFACTANT, AN ORGANIC MODIFIER, A DUAL MIXTURE OF NEUTRAL CHIRAL SELECTOR CONSISTS OF HYDROXYPROPYL-β-CYCLODEXTRIN (HP-β-CD) AND HYDROXYPROPYL-γ-CYCLODEXTRIN (HP-γ-CD) IN A RATIO OF 25 TO 29: 5 TO 1 IN MOLE.