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Methods for producing mushroom Agaricus bisporus without spores

机译:无孢蘑菇双孢蘑菇的生产方法

摘要

A method for determining if a culture of Agaricus bisporus contains a genetic determinant for at least one characteristic of absence of spores, selected from the group consisting no sporulation reduced sporulation, incomplete development of spores, incomplete release of spores and lamellae paler, this method comprising: facilitating fusion via anastomosis and plasmogamia between a first crop of Agaricus bisporus and a second crop of Agaricus bisporus, where the second crop is a homocarión selected from the group consisting of a culture Agaricus wild bisporus JB2, a wild growing Agaricus bisporus JB28, a culture derived from JB2 or JB28, a descendant of JB2 or JB28 culture, WQ, S-381, S-600, M1, 56B- 4186, TS5 s4 and descendants cultures or WQ derivatives, S-381, S- 600, M1, 56B-4186 and TS5 s4, and further identified as capable of transmitting germplasm to offspring at least one feature of absence of Espor as selected from the group consisting of no sporulation reduced sporulation, incomplete development of spores, spores and incomplete release paler lamellae; where if the hybrid strain of this merger has a characteristic expressed by a phenotype without spores, the first crop contains a genetic determinant for at least one characteristic of absence of selected spores from the group consisting no sporulation reduced sporulation, incomplete development of spores , incomplete release of spores and lamellae paler.
机译:一种用于确定双孢蘑菇培养物是否包含至少一种不具有孢子特征的遗传决定子的方法,该方法选自无孢子形成减少的孢子形成,孢子发育不完全,孢子释放不完全和薄片较淡,该方法包括:促进通过双孢蘑菇的第一批作物和双孢蘑菇的第二批作物之间的吻合和血丝虫融合,其中第二种作物是选自培养的野生双孢蘑菇JB2,野生生长双孢蘑菇JB28,双孢蘑菇JB28,来自JB2或JB28,JB2或JB28文化的后代,WQ,S-381,S-600,M1、56B-4186,TS5 s4和后代文化或WQ派生产品,S-381,S-600,M1的后代56B-4186和TS5 s4,并进一步确定为能够将种质传递给后代的至少一个特征,该特征选自没有孢子形成的组,该组织没有孢子形成减少了孢子形成,包括孢子充分发育,孢子和不完全释放的浅层片状;如果该合并的杂种菌株具有无孢子表型表达的特征,则第一批作物包含至少一个不存在孢子的遗传决定因素,这些特征包括无孢子形成,孢子形成减少,孢子发育不完全,不完全孢子的释放和片状的苍白。

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