Exosome extraction method and microfluidic exosome extraction device (Machine-translation by Google Translate, not legally binding)

摘要

Method of extracting exosomes according to their diameter, comprising a step of introducing a fluid sample comprising at least two populations of exosomes with a different diameter each in a microfluidic device (10) comprising at least one channel (1)) comprising at least one inlet (2) for the fluid sample and at least two holes (3) distributed along the channel (1) that allow exosome populations to exit and an outlet (5) for the fluid sample remaining, and means (6) to generate a temperature gradient in the channel (1); and a stage in which the fluid sample is flowed through the channel (1) while a temperature gradient is applied in the channel, causing the exit of each population through a different hole (3). Microfluidic device (10) for the application of the method. (Machine-translation by Google Translate, not legally binding)