Method to overcome the sensitivity to chemical modifications in the DNA of manipulated TALE DNA binding domains

摘要

A method for synthesizing a transcription activator type effector protein (TALE) to target a nucleic acid sequence comprising a methylated nucleic acid base, said method comprises assembling a plurality of TALE type repeat sequences, each of said sequences comprises a repetitive variable dirresiduo (RVD) specific for each nucleic acid base of said sequence, wherein the RVD that specifically targets the methylated nucleic acid base in said nucleic acid target sequence is selected of X *, where X represents an amino acid residue selected from the group of A, G, V, L, I, M, S, T, C, P, D, E, F, Y, W, Q, N, H , R and K and * represents a gap in a RVD position.