Methods of processing lignocellulosic biomass by single-stage autohydrolysis and enzymatic hydrolysis with c5 derivation and subsequent hydrolysis

摘要

A lignocellulosic biomass processing method comprising: - Providing soft lignocellulosic biomass feedstock, - Pretreatment of the feedstock to pH within the range of 3.5 to 9.0 in a single stage pressurized hydrothermal pretreatment with an MS content of at least 35% at temperatures between 160 and 200 ° C for residence times of less than 60 minutes, so that the pretreated biomass is characterized by having a xylan number of 10% or higher, - Separate the biomass pretreated in a solid fraction and a liquid fraction, - Hydrolyze the solid fraction with or without addition of supplemental water content of 15% DM or higher for a time between 24 and 150 hours using enzymatic hydrolysis catalyzed by a mixture of enzymes that it comprises endoglucanase, exoglucanase, B-glucosidase, endoxylanase, xylosidase and acetyl xylan esterase activities, and - Subsequently mix the separated liquid fraction and the hydro solid fraction After the at least 50% conversion of cellulose to glucose has been obtained and subsequent hydrolysis is carried out for a period of at least 6 hours, the xylooligomers in the liquid fraction are degraded to monomeric xylose by action of enzymatic activities that remain within the hydrolyzed solid fraction.