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METHOD FOR QUANTITATIVELY MEASURING INTERACTION BETWEEN MEMBRANE PROTEINS IN LIVING CELLS
METHOD FOR QUANTITATIVELY MEASURING INTERACTION BETWEEN MEMBRANE PROTEINS IN LIVING CELLS
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机译:定量测定活细胞膜蛋白之间相互作用的方法
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摘要
The present invention relates to a method for analyzing an interaction between a target membrane protein and a binding partner membrane protein for the target membrane protein in living cells. According to the present invention, in the method for analyzing an interaction between a target membrane protein and a binding partner membrane protein for the target membrane protein in living cells, the dissociation constant for the interaction can be calculated by treating cells to be analyzed with a capture probe for the target membrane protein to immobilize the target membrane protein on a substrate and measuring a change of the diffusion coefficient distribution of the binding partner membrane protein for the target membrane protein. Therefore, the interaction between the target membrane protein and the binding partner membrane protein for the target membrane protein can be quantitatively measured.
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