A method for improving adsorption of a polysaccharide-protein conjugate and a polyvalent vaccine preparation obtained therefrom

摘要

The present invention provides a method for preparing a stable polyvalent pneumococcal polysaccharide-protein conjugate vaccine preparation. The stable formulation exhibits an optimal adsorption rate (%) for each conjugate, where aggregation is either i) individually or separately adsorbed to the conjugate which otherwise exhibits a lower adsorption rate (%) by combined adsorption ii) neutral pH To the acidic pH; iii) a non-iv) formulation of the polysaccharide to a protein of from about 0.5 to about 1.4, by the use of a 6-day rush ton type turbine impeller.