Secretory protein detection chip And Secretory protein detection device for Disc disease research

摘要

The secretory protein detection chip for an intervertebral disc disease research according to an embodiment of the present invention comprises: an intervertebral disc chamber in which inflammation-treated intervertebral disc cells are cultured; Neural chambers in which neurons are cultured; A neural chamber connection channel in which cell migration between intervertebral cells and nerve cells is interrupted to connect intervertebral disc chambers and neural chambers to release secreted proteins from inflammatory disc cells; A first transfer channel branching in the nerve chamber connection channel, through which the secretory protein flows; And a FET-based sensor unit connected to the first movement channel and treated with a reactant that is antigen-antibody-conjugated with the target protein causing pain of the intervertebral disc among the secretory proteins, and senses the target protein by the change of electrical signal at the binding of the antigenic antibody Secretory protein is provided to the nerve chamber through the nerve chamber connection channel to stimulate the nerve cell and is provided to the FET-based sensor section via the first movement channel, and when the nerve cell is stimulated by the secretory protein, It is desirable to grow in the interconnect channel.