首页> 外国专利> A NEW CULTURAL ENVIRONMENT FOR THE REMOVAL OF UNDIFFERENTIATED STEM CELLS, AND ALSO FOR CLEANING AND OPTIMIZATION OF MYOCARDIAL CELLS

A NEW CULTURAL ENVIRONMENT FOR THE REMOVAL OF UNDIFFERENTIATED STEM CELLS, AND ALSO FOR CLEANING AND OPTIMIZATION OF MYOCARDIAL CELLS

机译:去除未分化干细胞以及清洁和优化心肌细胞的新文化环境

摘要

The present invention has as its object to find out new conditions that make it possible to induce cell death of non-cardiomyocytes or undifferentiated stem cells more completely and to select cardiomyocytes only. In order to achieve this object, there are provided in the present application: a cell culture medium for use in inducing cell death of undifferentiated stem cells, wherein the cell culture medium is free of glutamine in the amino acid profile; and also a method for inducing cell death of non-cardiomyocytes by performing cell culture in said cell culture medium. Further provided in this application are: a cell culture medium for use in selecting cardiomyocytes, wherein the cell culture medium is supplemented with lactate, pyruvate or a fatty acid, free of sugar, and free of glutamine in the amino acid profile; and also a method for selecting cardiomyocytes by culturing a mixture of cardiomyocytes and non-cardiomyocytes in said cell culture medium.
机译:本发明的目的是发现新的条件,使得有可能更完全地诱导非心肌细胞或未分化干细胞的细胞死亡,并仅选择心肌细胞。为了实现该目的,在本申请中提供了一种用于诱导未分化的干细胞的细胞死亡的细胞培养基,其中该细胞培养基的氨基酸谱中不含谷氨酰胺。以及通过在所述细胞培养基中进行细胞培养来诱导非心肌细胞的细胞死亡的方法。在本申请中还提供了:用于选择心肌细胞的细胞培养基,其中所述细胞培养基在氨基酸谱中补充有乳酸,丙酮酸或脂肪酸,无糖和无谷氨酰胺。以及通过在所述细胞培养基中培养心肌细胞和非心肌细胞的混合物来选择心肌细胞的方法。

著录项

相似文献

  • 专利
  • 外文文献
  • 中文文献
获取专利

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号