An enzyme—polymer conjugate which has at least one hydrolase enzyme immobilised thereon is provided. The conjugate has the structure of Formula I: wherein R1 is a hydrolase enzyme and R2 is OH or O-, or R1 is OH or O- and R2 is a hydrolase enzyme; i is an integer from 1 to 19; and n is an integer from 5 to 1000. The SMA polymer may be suitable for use in a biotechnological process or for protecting a surface from biofouling. Preferably the hydrolase enzyme is chosen from amylase, protease and galactosidase and the enzyme forms a covalent bond between a lysine group of the enzyme and a maleic anhydride group of the polymer. In an embodiment, some subunits are conjugated to amylase and others to protease. Preferably the conjugate is in the form of nanofibres, coatings, nanoparticles or microparticles.
展开▼
