ARTIFICIAL ANTIGEN PRODUCED USING PARTIAL SEQUENCE OF ENOLASE PROTEIN ORIGINATED FROM PLASMODIUM FALCIPARUM, AND METHOD FOR PRODUCING SAME

摘要

The present invention provides a bioactive peptide including a partial amino acid sequence of Plasmodium falciparum enolase, and having a molecular structure compatible with a specification setting for a GMP-compliant production process. Provided is a peptide having a structure in which two peptides each having (i) an amino acid sequence represented by: A01-Ala-Ser-Glu-Phe-Tyr-Asn-Ser-Glu-Asn-Lys-Thr-Tyr-Asp-Leu-Asp-Phe-Lys-Thr-Pro-Asn-Asn-Asp-A02 (SEQ ID NO: 1) or (ii) an amino acid sequence represented by: A03-Ala-Ser-Glu-Phe-Tyr-Asn-Ser-Glu-Asn-Lys-Thr-Tyr-Asp-Leu-Asp-Phe-Lys-Thr-Pro-Asn-Asn-Asp-Lys-Ser-Leu-Val-Lys-Thr-A04 (SEQ ID NO: 2) are linked by amide bonds between the respective carboxy termini of the two peptides and two amino groups of Lys in a linker peptide represented by (iii): Lys-A05-Cys-A06 and arranged in the form of a two-forked branch. Preferred is a peptide having a dimerized structure in which two of the above described peptides are linked by an S-S bond between the Cys residues in the linker peptide sequences included in the respective two peptides. (In the above (i), (ii), and (iii), A01 to A06 each represents an amino acid residue in a number of an arbitrary number including 0).