DEVICE FOR PARALLEL OLIGOMER SYNTHESIS, METHOD OF PARALLEL OLIGOMER SYNTHESIS AND USE THEREOF

摘要

Present patent application relates to a device for parallel oligomer synthesis comprising a centrifuge with a plurality of reactor holders configured to retain reactors at an angle and a plurality of S-trap outflow holders, a plurality of reactors having upper ends and lower ends, the lower ends comprising nozzles, the reactors being positioned at an angle in the reactor holders, a plurality of S-trap outflows having first ends and second ends, the first ends being connected to the nozzles of the lower ends of reactors, and the S-trap outflows being configured to work on a siphoning principle, a distribution rotor being provided with pre-activation compartments at its outer circumference, the pre-activation compartments being provided with grooves directed towards the outer edge of the distribution rotor, and configured to enable the transfer of contents from the pre-activation compartments of the distribution rotor to the reactors upon rotation of the distribution rotor, a lifting device being configured to enable the distribution rotor to move in vertical direction between an upper position and a lower position, a positioning device being configured to enable a rotation of the distribution rotor in the upper position independently from the centrifuge, a driving motor, the driving motor, the centrifuge and the distribution rotor having the same common axis, the driving motor being configured to enable a synchronized rotation of the centrifuge and the distribution motor in the lower position, an outflow channel being positioned at the outer circumference of the centrifuge, the second ends of the S-trap outflows being positioned in said outflow channel continuous groove, the outflow channel further comprising a plurality of outflow openings, a system of outflow pipes, the outflow pipes being connected to outflow openings of the outflow channel and being positioned below the centrifuge, a dosing device comprising a distribution valve, with a plurality of ports and a dosing pump being configured to measure the exact quantity of liquid reagents, the dosing pump being connected to the distribution valve, the distribution valve being connected to stock solutions of reactants and/or solvents and/or activating reagents and/or coupling reagents via plurality of ports of the distribution valve and via tubing, and a control device being provided with a timing protocol and connected to said driving motor, said dosing device and said lifting and positioning device. Present patent application further relates to a method of parallel solid-based peptide synthesis following the timing protocol of the device according to the invention and a use of the device for parallel oligomer synthesis according to the invention.