首页>
外国专利>
COMPOSITIONS AND METHODS USING CAS9 WITH ENHANCED SPACER ACQUISITION FUNCTION
COMPOSITIONS AND METHODS USING CAS9 WITH ENHANCED SPACER ACQUISITION FUNCTION
展开▼
机译:结合使用具有增强的空格获取功能的CAS9的组合物和方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
Provided are Cas9 enzymes that have mutations that enhance their properties, relative to un-mutated Cas9. The altered Cas9 enzymes exhibit i) an increased rate of spacer acquisition, or ii) increased cleavage efficiency of targets with NAG PAMs, or a combination of i) and ii). The altered Cas9 enzymes comprise an amino acid substitution of 1473 and K500 in a Streptococcus pyogenes or similar Cas9 enzyme. Also provided are polynucleotides, including expression vectors that encode the Cas9 enzymes, cells that contain the polynucleotides, and methods of making and using such cells. The disclosure includes tagging, or labelling bacteria, and for enhancing phage acquired immunity in bacteria, such as those used in industrial processes, including the food and beverage industry, such as the dairy industry. The food products are also included.
展开▼