Stromal cells from lymph nodes in patients with hematological disorders

摘要

PROBLEM TO BE SOLVED: To provide a culture system which can reproduce the microenvironment of lymphoproliferative diseases in order to elucidate the microenvironment of lesions and to establish an effective treatment method.SOLUTION: A method for preparing human stromal cells originating from lymph nodes of patients with lymphoproliferative diseases comprising the steps of: (1) removing blood cell components from a biopsy sample collected from a lymph node of a patient with a lymphoproliferative disease; and (2) culturing in a nutrient medium to which serum has been added.SELECTED DRAWING: Figure 1