Reliable fluorescence correction method for two-color measurement fluorescence system

摘要

Methods and devices use in two-color measurement systems. The methods and devices include methods of making corrections, methods of calculating correction factors, fluorescence scanners, and microarray chips. The said methods and devices enable a user to correct fluorescence intensities for errors caused by the occurrence of FRET and/or cross-talk when two fluorophores are used in two-color fluorescence arrays.