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Carbon-detected NMR for mapping binding sites in intrinsically disordered regions of a protein
Carbon-detected NMR for mapping binding sites in intrinsically disordered regions of a protein
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机译:碳检测的NMR用于绘制蛋白质内在无序区域中的结合位点
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摘要
Carbon-detected NMR is well-suited for mapping binding sites in intrinsically disordered regions of a polypeptides, and for mapping of binding motifs in intrinsically disordered regions with single-residue resolution. Provided are methods of carbon-detected NMR for determining the amino acids that mediate the interaction between an intrinsically disordered polypeptide or protein, or an intrinsically disordered region of a polypeptide, and a biomolecule such as another polypeptide or a nucleic acid.
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