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Binary vectors with minimized biosafety concerns and high transformation rates by engineered plant-derived transfer-DNA
Binary vectors with minimized biosafety concerns and high transformation rates by engineered plant-derived transfer-DNA
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机译:通过工程化植物衍生的转移DNA最小化生物安全问题和高转化率的二元载体
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摘要
An engineered plant-derived transfer (P-)DNA was designed and constructed based on a couple of T-DNA homologous sequences in sugar beet genome. Plant transformation efficacy of the engineered P-DNA was analyzed compared to conventional T-DNA in two independent systems, stable transformation of tobacco plant and sugar beet hairy roots. The outcomes demonstrated that plant transformation is directed well by vectors carrying the engineered P-DNA with higher efficiency than the conventional binary vector in both experimental systems. This vector was further improved by adding two matrix attachment regions within P-DNA. This new vector was even more efficient in gene transfer, higher than conventional binary vector.
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