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SCARLESS DNA ASSEMBLY AND GENOME EDITING USING CRISPR/CPF1 AND DNA LIGASE
SCARLESS DNA ASSEMBLY AND GENOME EDITING USING CRISPR/CPF1 AND DNA LIGASE
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机译:使用CRISPR / CPF1和DNA连接酶进行无痕DNA组装和基因组编辑
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摘要
The disclosure describes a scarless DNA assembly and genome editing methodology termed “CLIC” (CRISPR and Ligase Cloning), which utilizes a CRISPR/Cpf1 complex and DNA ligase to perform programmable gene editing and nucleotide assembly. The CLIC process is highly amenable to applications in vitro for the scarless assembly of a plurality of DNA parts simultaneously or in vivo for the site-specific insertion of one or more DNA molecules into the host genome.
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