CONSTRUCT OF NUCLEIC ACIDS COMPRISING A PROMOTER LINKED TO A NUCLEOTIDE SEQUENCE ENCODING THE PRECURSOR OF miRNA 319e OF V. VINÍFERA; METHOD FOR REGULATING THE EXPRESSION OF A TARGET SEQUENCE IN A TARGET CELL AND ITS USE IN POST-TRANSCRIPTIONAL GENE SILENCING OF A TARGET SEQUENCE

摘要

A nucleic acid construct comprising a promoter operably linked to a nucleotide sequence coding for the precursor of Vitis vinifera miRNA 319e capable of generating an amiRNA hairpin structure that includes a complementary hypervariable sequence between 60-100% to a target sequence. A method of regulating the expression of a target sequence in a target cell comprising introducing the nucleic acid construct into a target cell, and using the construct in the post-transcriptional gene silencing of a target sequence in a constitutive and stable manner.