new pcr sequencing method and its use in hla genotyping.

摘要

pcr sequencing method and use in hla genotyping - the invention provides a pcr sequencing method, where the combination of primary indices, an incomplete dna shear strategy and a second generation sequencing technique (sequencing technique) may result in the length of pcr products that can be sequenced by a sequencer greater than the maximum sequencing length of the sequencer while making full use of the features of the second generation sequencing technique such as high yield and low cost, thereby greatly expanding its applicable scope. furthermore, the present invention also provides primary indices for the pcr sequencing method and the use of the method in genotyping, particularly in hla analysis, and also provides the primary pcrs used, particularly the primary pcrs for the hla-a genes, b, hla-c and hla-dqb1.