Two pairs of starter oligonucleotides for detecting the presence of alleles of the dominating or recessive immunity gene to crown rust Pc39 in the genome of common oat (Avena sativa L.), combination of two starter pairs and method for detecting of the of gene Pc39 alleles system

摘要

reported are two pairs of oligonukleotydowych primers.namely: silicodart2 _ f1 and silicodart2 _ r1 for detecting the dominant allele of gene resistance to rust koronową pc39 in oat plants of sequences 1 and 2 provided on the list and sequencedart2 _ f and dart2 _ r to detect allele gene in plants recesywnego pc39 oats of sequences 3 and 4 provided on the sequence. the subject invention is also a combination of these pairs.object of the application is also the dominant allele of the gene identification resistance to rust koronową pc39 in oat plants,in which the polymorphic dna fragment coupled with the gene amplifikowany in pcr reaction using a pair of primers, which are detection of amplifications.a pair of primers and primers sequences of a pair of oligonukleotydowych no 1 and 2 provided on the sequences using marker silicodart2.as a result of the pcr reaction amplifikowany is dna fragment length of 42 pairs of sequence number 5 presented in the sequence.however, the identification recesywnego allele gene pc39 in oat plants, is according to the wayin which the polymorphic dna fragment coupled with the gene amplifikowany in pcr reaction using a pair of primers, which are detection of amplifications.the pair of primers is a pair of oligonukleotydowych primers sequences of no 3 and 4 provided on the sequences using marker dart2.as a result of the pcr reaction amplifikowany is dna fragment length of 420 pairs of sequence shown in the sequence number 6.