Method for high-resolution fluorescent imaging, using nanoluminophores doped with ions of lanthanides, application of the method for high-resolution fluorescent imaging below the limit of diffraction and the measuring system that executes the method

摘要

The purpose of the notification is to provide a high -resolution luminescent image using luminescent markers in the form of nano -dimensional luminescent markers mixed with rare earth ions, and to colour, illuminate the test structure, record the signal, create a map of the object characterised by it, that the phase -induced luminescent markers are dielectric nano -dimensional luminescent markers mixed with rare earth ions and the laser beam inducer has a wavelength of UV (350 -400 nm) or visible light (400 -800 nm) or near infrared (800 -1300 nm),and nano -dimensional luminescent markers have a diameter of 5 to 50 nanometers, the concentration of mixtures being 0,01 to 100%,where the sum of the concentration of all rare earth ion mixtures is 0,01 to 100%,where rare earth ions have metastable levels characterised by long life periods of levels awakened from 10 -6 to 10 -2 s. The second subject of the notification i s the use of the described method of measurement and nano -dimensional luminescent markers for high resolution imaging below the limit Division. Another subject of the notification is the measurement system implementing the method.