monomeric and dimeric forms of adiponectin receptor fragments and methods of use

摘要

A method of determining the progression, initiation, or efficacy of diabetes treatment characterized by an adipocyte imbalance in a patient, comprising: determining a level of a peptide in a sample of biological fluid obtained from said patient; wherein said peptide is selected from the group consisting of: a first heterodimer comprising a first grouper unit and a second grouper unit, wherein said first grouper unit has at least approximately 90% identity with SEQ ID NO: 5 and wherein said second mer unit has at least about 90% identity with SEQ ID NO: 6, and wherein said first mer unit and said second mer unit are joined via a disulfide bond; a first homodimer comprising a first grouper unit and a second grouper unit, wherein said first grouper unit has at least approximately 90% identity with SEQ ID NO: 6 and wherein said second grouper unit is at least approximately 90% identity with SEQ ID NO: 6, and wherein said first mer unit and said second mer unit are joined via a disulfide bond; a second heterodimer comprising a first grouper unit and a second grouper unit, wherein said first grouper unit has at least approximately 90% identity with SEQ ID NO: 1 and wherein said second grouper unit is at least approximately 90% identity with SEQ ID NO: 6, and wherein said first mer unit and said second mer unit are linked through a disulfide bond; a third heterodimer comprising a first grouper unit and a second grouper unit, wherein said first grouper unit has at least approximately 90% identity with SEQ ID NO: 2 and wherein said second grouper unit is at least approximately 90% identity with SEQ ID NO: 6, and wherein said first mer unit and said second mer unit are linked through a disulfide bond; a dimer of said first heterodimer; a dimer of said first homodimer; a dimer of said second heterodimer; a dimer of said third heterodimer; and correlating the level of said selected peptide with said progression of onset of or efficacy of diabetes treatment.