Process for the production of a hybrid structure consisting of microfibres and coupled silk fibroin nanofibers, hybrid structure obtained in this way and its use as an implantable medical device

摘要

Process for the production of a hybrid structure made of microfibers and nanofibers of silk fibroin coupled together, comprising the following steps: a) preparation of one or more parts made of microfibrous fibroin; b) preparation of one or more parts made of nanofibrous fibroin; c) treatment of said one or more parts of nanofibrous fibroin and of said one or more parts of microfibrous fibroin, separately or after coupling, with a solvent for fibroin and / or with a solution comprising fibroin dissolved in a solvent; c ') if in step c) the nanofibrous and microfibrous parts have been treated separately with a solvent for fibroin and / or with a solution comprising fibroin dissolved in a solvent, coupling of said parts; d) consolidation of the hybrid microfibrous / nanofibrous structure obtained in stage c) or in stage c ') by heat treatment at a temperature between 10 ° C and 150 ° C, for a time between 1 minute and 24 hours; e) removal of the solvent by washing with water or a water-alcohol mixture at a temperature between 10 ° C and 100 ° C, or by evaporation at a temperature between 10 ° C and 100 ° C, possibly under vacuum, in which the solvent used in step c) is selected from formic acid, 1,1,1,3,3,3-hexafluoro-2-propanol, trifluoroacetic acid, hexafluoroacetone, N-methylmorpholine N-oxide, ionic liquids, calcium chloride mixtures20 ethanol -water, mixtures of calcium nitrate-methanol-water, aqueous solutions of lithium salts and mixtures between these solvents and / or with water.