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METHOD FOR DETECTING THE PRESENCE OF ONE OR MORE BACTERIAL TOXINS IN A BIOLOGICAL FLUID USING LIPOSOMES
METHOD FOR DETECTING THE PRESENCE OF ONE OR MORE BACTERIAL TOXINS IN A BIOLOGICAL FLUID USING LIPOSOMES
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机译:脂质体检测生物流体中一种或多种细菌毒素的方法
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摘要
The present invention relates to a method for detecting the presence of one or more bacterial toxins, capable of binding to cell membranes, in biological fluid wherein the method comprises: (i) incubating the biological fluid with a plurality of liposomes, wherein the liposomes comprise a lipid capable of binding to said one or more toxins, to provide one or more liposome-toxin conjugate; (ii) incubating said conjugates with at least one type of antibody bound to a label to provide one or more conjugate-antibody complex; wherein each type of antibody in the mixture is specific for one of the bacterial toxins whose presence is to be detected; and (iii) analysing said complexes in order to detect the presence of one or more bacterial toxins capable of binding to cell membranes. Further aspects of the invention relate to a conjugate-antibody complex useful in the methods of the invention and a kit useful for performing the method of the invention.
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