SNP MARKER RELATED TO LOW-SALT TOLERANCE CHARACTERISTIC OF LITOPENAEUS VANNAMEI, AMPLIFICATION PRIMER THEREFOR, AND APPLICATION THEREOF

摘要

Disclosed are an SNP marker related to the low-salt tolerance characteristic of Litopenaeus vannamei, an amplification primer therefor, and an application thereof. A primer is designed on the basis of the mRNA sequence (GenBank: KF765670.1) of the Na,K-ATPase α subunit of Litopenaeus vannamei obtained from NCBI, and the genomic DNA of low-salt tolerant and low-salt intolerant Litopenaeus vannamei are used as templates respectively for PCR amplification to obtain PCR products with a length of 533 bp respectively. The sequencing results are compared and analyzed. A total of 11 SNP sites are detected in this sequence. An SNP marker related to low-salt tolerance is identified by correlative analysis of the genotypes of these templates at each SNP site.