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Analytical method for quanitification of viable bacteria contained in microbiota restoration therapy (MRT) compositions
Analytical method for quanitification of viable bacteria contained in microbiota restoration therapy (MRT) compositions
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机译:微生物群恢复治疗(MRT)组合物中所含活菌定量的分析方法
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摘要
Quantification of the viable bacterial microorganisms in a drug product for delivery via a gastro-nasal tube, an enema and/or a capsule or tablet. A molecular-based approach, such as PMA (propidium monazide)-qPCR (quantitative polymerase chain reaction) assays may be useful for quantification of viable bacteria. By utilizing PMA treatment in combination with qPCR, the number of viable bacterial cells in the sample can be determined.
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