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METHOD AND TECHNICAL SYSTEM FOR CULTIVATING APHID-RESISTANT TOBACCO AND WHEAT

机译:种植抗蚜虫烟草和小麦的方法和技术系统

摘要

Provided is a method for cultivating aphid-resistant tobacco and wheat. The method comprises: inhibiting the expression of a specific gene or specific gene fragment in an initial plant to obtain an aphid-resistant transgenic plant. The specific gene fragment is the aphid-specific target gene 22544 isolated from the aphid's intestinal tract, with the nucleotide sequence thereof as shown in sequence 1, and the specific gene is a gene having the nucleotide sequence as shown in sequence 1. Also provided is a system for cultivating the aphid-resistant transgenic plant, comprising an interference fragment or interference vector that inhibits the expression of the specific gene or specific gene fragment in the initial plant. The transgenic plant obtained by introducing the target gene 22544 as the target, wherein the expression of same is to be inhibited, into the interference fragment or interference vector exhibits a good aphid-resistant phenotype.
机译:提供了一种栽培抗蚜虫的烟草和小麦的方法。该方法包括:抑制起始植物中特定基因或特定基因片段的表达以获得抗蚜虫的转基因植物。该特定基因片段是从蚜虫的肠道分离的蚜虫特异性靶基因22544,其核苷酸序列如序列1所示,并且该特定基因是具有核苷酸序列如序列1所示的基因。一种用于培养抗蚜虫的转基因植物的系统,其包含抑制特定基因或特定基因片段在初始植物中表达的干扰片段或干扰载体。通过将靶基因22544作为靶而导入其中抑制其表达的靶基因而获得的转基因植物表现出良好的抗蚜虫表型。

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